REVIVO BioSystems' technology combines the principles of microfluidics with those of biological transport phenomena and tissue engineering.
It exploits fluid flow control and miniaturization for culturing human tissues and automating the testing procedures.
Our organ-on-chip systems mimic the function of the blood flow and create a unique micro-environment for epithelial tissue models that require to be maintained at the air-liquid interface, ultimately establishing realistic conditions and in vivo-like tissue properties.
Our platform automates the testing procedures and it is the ideal tool for cost-effective ex vivo and in vitro screening on epithelial tissue models.
REVEx™ chip
ReleGO™
Automated Fraction Collector
We are unique.
Our platform recreates in vivo-like conditions.
We closely mimic subcutaneous blood flow.
We enable easy access to the tissue surface.
Application of [multiple/repeat] test substances is easier than ever.
Our platform is automated and significantly saves manpower.
Our platform is dynamic, allowing to make setup changes to the testing protocol.
REVIn™ Plate
Our Proprietary 4D Human Skin Models
Superior barrier properties
Full thickness & morphology
Realistic 'subcutaneous' blood flow
Viable skin for up to 21 days
Reach your market faster, more cost efficiently and more ethically.
Real human skin - no more animal cruelty
Realistic & accurate so less overall testing
4X less Biomaterials by miniaturisation
10X less manpower to operate testing
Case Studies
Skin culture on REVIn™ chip
Full thickness skin equivalents with enhanced epidermal morphogenesis and barrier function could be reconstructed within the microfluidic chip.
Skin-on-chip
Static culture
Human skin
Involucrin and the late differentiation marker loricrin are more intense and continuously distributed in the granular layers of the skin-on-chip epidermis
Strong deposition of collagen type IV in the basement membrane zone of the skin-on-chip (similar to native human skin) compared to the patchy expression in static cultures
Integrin-β1 expression at the basement membrane zone and at the apico-lateral membranes of polarized basal keratinocytes is similar to normal human epidermis
Scale: 50µm
TEER: the skin-on-chip equivalents (SoCE) mean resistance is twice as large as that measured in static culture skin equivalents (static SE)
Permeation: the SoCEs showed a 1.6-fold lower mean caffeine cumulative amount and a 1.6-fold lower permeability coefficient compared to the static SEs.
Biopsies on REVEx™ & ReleGO™
When tissue explants (e.g. skin) or simulants are loaded in the microfluidic chip, the system provides the most realistic tissue model setup available because of the mimicry of the blood flow and microenvironment. It enables a high level of automation of the ex vivo testing procedure, higher precision in permeation experiments and reduces unstirred water layers.
REVEx™ chip
ReleGO™
Automated Fraction Collector
The REVEx™ chip is a six-chamber microfluidic device where six tissue (e.g. skin) explants or simulants can be loaded, cultured, and used for testing in a miniaturized flow-through setup.
ReleGO™ is an innovative automated fraction collector that maintains two REVEx™ chips at controlled temperature and enables smart integration with electrodes and sensors.
Tunable flow rate and temperature to mimic in vivo-like conditions
Automated fraction collections directly in 96 well plates
User-friendly programming interface (duration, sampling intervals, etc.)
Includes a fully automated permeation protocol
Just a 0.5 cm specimen per replicate
Up to 12 replicates per run
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