REVIVO BioSystems' technology combines the principles of microfluidics with those of biological transport phenomena and tissue engineering.

 

It exploits fluid flow control and miniaturization for culturing human tissues and automating the testing procedures.

Our organ-on-chip systems mimic the function of the blood flow and create a unique micro-environment for epithelial tissue models that require to be maintained at the air-liquid interface, ultimately establishing realistic conditions and in vivo-like tissue properties.

Our platform automates the testing procedures and it is the ideal tool for cost-effective ex vivo and in vitro screening on epithelial tissue models.

REVEx™ chip

ReleGO

Automated Fraction Collector

We are unique. 

Our platform recreates in vivo-like conditions.

We closely mimic subcutaneous blood flow.

We enable easy access to the tissue surface.

Application of [multiple/repeat] test substances is easier than ever.

Our platform is automated and significantly saves manpower.

Our platform is dynamic, allowing to make setup changes to the testing protocol.

REVIn™ Plate

Our Proprietary 4D Human Skin Models

Superior barrier properties

Full thickness & morphology

Realistic 'subcutaneous' blood flow

Viable skin for up to 21 days

Reach your market faster, more cost efficiently and more ethically.

Real human skin - no more animal cruelty

Realistic & accurate so less overall testing

4X less Biomaterials by miniaturisation

10X less manpower to operate testing

Case Studies

Skin culture on REVIn chip

Full thickness skin equivalents with enhanced epidermal morphogenesis and barrier function could be reconstructed within the microfluidic chip.

Skin-on-chip

Static culture

Human skin

Involucrin and the late differentiation marker loricrin are more intense and continuously distributed in the granular layers of the skin-on-chip epidermis

Strong deposition of collagen type IV in the basement membrane zone of the  skin-on-chip (similar to native human skin) compared to the patchy expression in static cultures

Integrin-β1 expression at the basement membrane zone and at the apico-lateral membranes of polarized basal keratinocytes is similar to normal human epidermis

Scale: 50µm

TEER: the skin-on-chip equivalents (SoCE) mean resistance is twice as large as that measured in static culture skin equivalents (static SE)

Permeation: the SoCEs showed a 1.6-fold lower mean caffeine cumulative amount and a 1.6-fold lower permeability coefficient compared to the static SEs.

Biopsies on REVEx & ReleGO

When tissue explants (e.g. skin) or simulants are loaded in the microfluidic chip, the system provides the most realistic tissue model setup available because of the mimicry of the blood flow and microenvironment. It enables a high level of automation of the ex vivo testing procedure, higher precision in permeation experiments and reduces unstirred water layers.

REVEx™ chip

ReleGO

Automated Fraction Collector

The REVEx chip is a six-chamber microfluidic device where six tissue (e.g. skin) explants or simulants can be loaded, cultured, and used for testing in a miniaturized flow-through setup.

ReleGO is an innovative automated fraction collector that maintains two REVEx™ chips at controlled temperature and enables smart integration with electrodes and sensors.

Tunable flow rate and temperature to mimic in vivo-like conditions

Automated fraction collections directly in 96 well plates

User-friendly programming interface (duration, sampling intervals, etc.)

 

Includes a fully automated permeation protocol

 

Just a 0.5 cm   specimen per replicate

 

Up to 12 replicates per run

2

*µFPA [MicroFluidic Permeation Array] is the microfluidic chip comprised in our REVEx™  platform.

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