ReleGO™ & REVex™ FAQ
Q: For how long can skin explants & other tissues be maintained viable on ReleGO ?
A: State-of-the-art ReleGO and our microfluidic chip, REVex create a dynamic and continuous flow of media. This system mimicks the natural function of blood flow beneath the skin tissues, keeping the skin tissues and other tissues fresh for up to two times longer in duration than traditional methods. This means you can achieve better flexibility, improved reproducibility, more accurate results and more time for analysis for your ex vivo and in vitro experiments.
Q: Besides skin explants, what other types of skin tissues can be used on ReleGO?
A: What makes ReleGO & REVex chip easy to adopt is that they are fully compatible with a wide range of skin models. ReleGO & REVex have been tested to be fully compatible with a wide variety of skin tissue models including real human skin, commercially available organotypic skin cultures, animal skin and silicon membranes, making it easy and quick for you to adapt to current project needs.
Q: Can we use ReleGO & REVex for other tissues besides skin?
A: ReleGO & REVex are not only capable of automating safety, efficacy, and permeation preclinical studies for skin tissues, but they also exhibit compatibility with other tissue types. Our microfluidics technology platform provides a highly controlled and dynamic environment, which can simulate realistic conditions for a range of tissues, including but not limited to oral mucosa, hair & scalp, liver, lung, and gut tissue. You can confidently explore the potential applications of ReleGO and REVex for a variety of research areas beyond skin tissue analysis.
Q: How long can you run an experiment on ReleGO?
A: The experimental duration can vary based on multiple factors such as the type of tissue used, experimental setup, and the particular research inquiry being pursued. On ReleGO, you can easily program the test period to range from several hours to an extended continuous duration of 58 days.
Q: What are the minimum and maximum ReleGO flow rates? How do we choose flow rates?
A: The minimum and maximum allowable flow rates of the ReleGO system are 0.5 to 120 uL/min. However, in practice for ex vivo and in vitro studies, a flow rate of 1-10 uL/min is more commonly used. As an example, we recommend a flow rate of 4 uL/min for permeation studies on the ReleGO. Moreover, ReleGO has been programmed to alert users if the selected flow rate is too high for your chosen experimental duration, time intervals, and type of collection plate.
Users have the flexibility to explore various flow rate settings to customise their 4D ex vivo & in vitro experiments.