Next-generation In Vitro Permeation Testing (IVPT)
Why conduct a permeation test?
Percutaneous adsorption of molecules is a critical step in the design and development of novel formulations in skincare and pharmaceutical research. Skin permeation can be conducted in vitro for evaluating topical formulations and dermal or transdermal drug delivery systems, to verify the bioavailability of active ingredients and estimate their effectiveness.
In vitro skin absorption and dermal penetration tests use human skin explants (ex vivo models), reconstructed human skin equivalents (in vitro models), or synthetic membranes.
Traditional technology for permeation studies
Current permeation testing is mainly conducted on Franz diffusion cells, but their limitations, such as high sample dilution, tedious manual operations, and large skin sample requirements, can hinder their efficacy, and call for alternative methods.
Overcoming the limitations of current state-of-art testing
REVIVO BioSystems has overcome the limitations of diffusion cells apparatuses by developing an automated testing platform (ReleGO™) that uses miniaturized, dynamic flow-through microfluidic devices (REVex™ chips) for permeation studies and in vitro release testing (IVRT).
Better data means better insights
REVex™ chip has been extensively tested against the Franz Diffusion system, and results have proven that it achieves significantly higher precision when conducting dermal permeation experiments.
*Coefficient of Variation measured during permeation experiment on synthetic membranes to validate the design of the microfluidic device versus Franz cells
REVex™: The Chip That Plays Well With All Skin Types
REVex™ chip has been engineered to be fully compatible with a wide variety of skin tissue models available on the market including real human skin, commercially available organotypic skin cultures, and synthetic membranes.
Automated permeation assays can be conducted using skin equivalents, real human skin explants and synthetic membranes. Infinite or finite dose can be used.
Figure 1. Example of an infinite dose study using the automated ReleGO™ platform and the REVex™ chips. Cumulative amount profiles for an infinite dose of caffeine penetrating through different types of skin explants and simulants) placed in REVex™ chips and variations according to the models.
Get detailed insights on your active ingredients' skin distribution and penetration with our specialized services
Our expert team at REVIVO BioSystems can provide you with the specialized services you need to screen, select, and analyze your active ingredients and formulations for optimal penetration and targeted delivery. With our cutting-edge technology and years of experience, we can help you gain detailed insights into your formulations' skin distribution and compare them to market leaders.
Source: Alberti M, Dancik Y, Sriram G, Wu B, Teo YL, Feng Z., Bigliardi-Qi M, Wu RG, Wang ZP, Bigliardi P. Multi-chamber microfluidic platform for high precision skin permeation testing. Lab Chip 2017;17:1625-34.
Wound healing with 4D tissue model
Wound Healing Skin Models: A Powerful Tool for Developing New Wound Treatments
Wound-healing skin models are used to study the process of wound healing and to test potential therapeutic interventions for acute and chronic wounds.
These models are designed to mimic different stages of wound healing in vitro, including inflammation, cell migration, proliferation, and extracellular matrix deposition, providing a controlled and reproducible environment to test the efficacy of wound healing products, such as dressings, creams, and gels.
Conventional non-dynamic skin models
The drawbacks of traditional static skin models for wound healing are that they cannot simulate the dynamic environment of a living tissue, such as blood flow, cell movement, and extracellular matrix remodeling, which are critical for wound healing.
ReleGO™ Automated Fraction Collector for Accurate Wound Healing Kinetics
The ReleGO™ automated fraction collector provides a dynamic system that can track changes in the same tissue over time, which eliminates inter-tissue variability. Our microfluidic platform can be used to easily determine the kinetic of wound healing, which is crucial for treating chronic conditions like type 2 diabetes.
It is easy to determine whether a treatment accelerates healing by monitoring a marker that is secreted by the skin when its barrier function is compromised
When a wound occurs, the various layers of the epidermis are stripped away, leaving the dermis exposed to the air. At this point, the skin promptly releases a wound marker as part of the healing process. As the epidermis gradually covers the surface during the healing process, the amount of wound marker decreases until the healing is fully complete. By tracking the secretion of this marker over time, we can gauge the rate of healing and determine whether a particular substance or condition can speed up or slow down the process.
Chronological aging model
Unveiling the Secrets of Aging: The Importance of Aging Models
Aging skin models are a critical component of the scientific toolkit for understanding the complexities of ageing, and for developing new treatments and interventions to improve skin health and appearance. In addition, a skin aging model can aid in the development of innovative skincare products, allowing scientists and manufacturers to test the efficacy of different formulations on aged skin.
Traditional models for skin aging
Current state-of-the-art skin ageing models rely heavily on the concept of oxidative stress. While this approach has yielded significant insights into the mechanisms of skin ageing, it has certain drawbacks. One of the major limitations of oxidative stress-based models is that they oversimplify the complex interplay of various biological processes involved in skin ageing. For instance, oxidative stress-based models tend to overlook the contribution of non-ROS-related factors to skin ageing, such as chronic inflammation, altered gene expression, and impaired cellular signaling pathways.
Advancing Understanding of Skin Aging with 4D organ-on-a-chip technology
REVIVO BioSystems utilizes a 4D tissue model called REVskin™ to simulate tissue ageing and explore treatments to protect skin cell proliferation and stemness. Utilizing the perfusing system, the proprietary ageing cocktail was administered to skin tissues to simulate chronological ageing. Unlike other models, this process does not rely on the traditional oxidative stress system and does not result in any observable toxicity or stress, as indicated by negative results from both IL-1α and LDH assays. By monitoring the release of markers in the simulated blood flow, this model allows for the effective testing of topically or systemically administered anti-ageing compounds. This non-oxidative based approach is more closely aligned with natural physiology and can only be achieved efficiently using a perfusion system.
The impact of a chronological ageing simulator on a reconstructed tissue composed of cells from a donor aged 4 years old was examined. Results showed that after 7 days of ageing induction, a notable reduction in epidermal thickness was observed, accompanied by a significant decrease in Ki67 and Lamin B1 staining. Ki67 is an indicator of actively dividing cells, while Lamin B1 is a marker associated with youthful characteristics.