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Are 4D In-Vitro Platforms the Game-changer in Understanding Skin Ageing & Product Performance?

[January 2024 Edition]

How a 4D in vitro platform compares to 3D

In the ever-evolving landscape of in vitro research, the transition from 3D to 4D is bound to become a paradigm shift. Traditionally, 3D tissue models and testing platforms have represented the most advanced systems for safety and efficacy assessments in vitro. However, REVIVO BioSystems’  “4th dimension”, marked by the mimicking of the blood flow, offers a revolutionary leap in understanding product performance over time.

The dynamic nature of our 4D in vitro platform transcends the limitations of static 3D models, providing a more comprehensive perspective that opens up new horizons for cosmetic and pharmaceutical innovation. 

The summary table below highlights the strengths of 4D methodologies with respect to conventional 3D ones.

Greater insights into skin ageing with our unique

Chronological Ageing Model 

In vitro skin models help us to understand the mechanisms of ageing, advancing the development of innovative treatments and skin care interventions. 

While informative, traditional in vitro models present limitations in reproducing natural biological expressions of significant proteins and gene expressions. This can hinder their ability to provide realistic insights into skin-related phenomena. 

With REVIVO BioSystems’ 4D in vitro tissue models that leverage its dynamic organ-on-a-chip technology, these skin models closely resemble native human skin where significant proteins and genes, closer to natural levels in native tissues, are expressed. We possess a skin model capable of mimicking chronological aging independently of oxidative stress, offering a more nuanced insight into the aging process of the skin.

Our 4D skin models are ready in a 'healthy' and 'youthful' state, allowing the aging process to unfold over time. Why is this a game-changer? Following the ageing process, it is easy to observe significant reduction in epidermal thickness in the aged 4D skin model, and noticeable significant changes to the expression of a youth marker and proliferation. In contrast, when skin models are reconstructed in static  cell culture inserts, they already exhibit certain age-related characteristics (e.g. low expression of youth and proliferation markers). As a result, their potential to provide an appropriate and accurate model for distinct anti-ageing product claims can be limited. 

The analogy diagram and histological analysis below give a visual representation of these differences. 

Explore further our Chronological Ageing Model and its applications on our website

For enquiries, reach out to our team of experts at

To take advantage of our special 10% discount on your first testing service order with us, click below: 

Upcoming Conference

Advances in 3D Cell Culture | 29 Jan - 3 Feb, 2024 |  Mumbai, India

“Organ-on-a-chip for preclinical and biological research” Session Talks:

🗓️ Save the date: February 3, 2024

📍 Venue: Taj The Trees, Mumbai, India

🕘 9.00 - 9.35am Talk by Prof.  Sriram Gopu, Scientific Advisor/Co-Founder at

REVIVO BioSystems

Talk title: “Microfluidic Organ-on-a-Chip Strategies for Dental, Oral & 

Craniofacial Research”

🕤 9.35 - 10.05am Talk by Dr. Massimo Alberti, CEO at REVIVO BioSystems

Talk title: “4D Tissue Models for next generation safety & efficacy studies”


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